IMB - Queensland Facility for Advanced Genome Editing (QFAGE)
UQ life sciences and biomedical researchers now have a dedicated, in-house facility providing access to the rapid, precise and low-cost gene editing technology that is revolutionising biological research, agriculture, medicine and biotechnology.
The Institute for Molecular Bioscience (IMB) Queensland Facility for Advanced Genome Editing (QFAGE) provides expert genetic modification services using CRISPR/Cas9 genome editing technology in mice. Possible modifications include exon or gene deletion, other large genomic deletions, generation of small, specific changes (e.g. point mutations, small specific insertions/deletions), epitope tagging, generation of conditional (“floxed”) alleles, and insertion of large DNA fragments or reporters.
QFAGE also generates transgenic (TG) mice by pronuclear injection. Using next-generation piggyBac constructs, QFAGE is able to produce transgenic embryos at extraordinarily high efficiencies of up to 85 per cent. Efficiency is so high that analysis of phenotype no longer depends on generating lines, but can be done transiently in injected embryos, providing dramatic time and cost savings.
The facility offers a flexible service to assist life sciences and biomedical research groups in reaching their functional genomics goals.
Services are overseen by IMB Group Leader Professor Peter Koopman, whose lab has successfully produced dozens of TG and CRISPR/Cas9 models, and the facility is managed by Dr Johnny Huang, who has several years of experience managing a commercial mouse TG and knockout mouse facility. The team’s combined experience assures a quality service that maximises experimental success.
Visit the website to find out more or book an appointment to discuss your project.