Genetic transformation in the era of transformation
TALENs and CRISPR/Cas9 are powerful genome editing systems that have been used to generate targeted changes for direct modification of endogenous genes in an increasing number of plant species. Researchers at Iowa State University Crop Bioengineering Consortium have been engaged actively in the development of a public sector genome editing platform. Here an Agrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis is described. This system was evaluated for its mutagenesis frequency and inheritability using maize genes. When crossed with a wild type maize line, null segregants carrying only the desired mutant alleles but free of CRISPR reagent transgene could be generated in as early as T1 progeny.
Delivery of proteins instead of protein-encoding DNA fragments into plant cells is of particular interest for genome editing because it avoids DNA (transgene) integration into the genome and generates precisely modified non-transgenic plants. The transience of the proteins in the cells can be advantageous for applications in which long term expression of transgenes is not desired. We report here an effective gold-plated mesoporous silica nanoparticle (Au-MSN) platform for co-delivery of proteins and nucleic acid (e.g., repair template or guide RNA) to plant tissues using a biolistic particle bombardment method. Au-MSN with large average pore diameters (10-nm) are shown to deliver and release marker proteins and plasmid DNA to the same cell after bombardment. We also demonstrate that the Au-MSN can be used to deliver recombinase, leading to site-specific, heritable edits in a plant genome.
The plant research community is moving toward a number of new breeding technologies that represent options for increased innovation and which may find greater public and regulatory acceptance over the use of transgenic approaches. Current regulatory aspects of genome-edited crops in the US will be discussed.st treatments for fungal infections in humans.
Professor Anderson AO will describe why the novel plant defensin HXP124 is an excellent candidate for developing a fast, effective topical treatment for fungal nail infections (onychomycosis).
Professor Kan Wang earned a BS in Biochemistry from Fudan University in Shanghai, China. She was sponsored first by the Chinese government then by the Rockefeller Foundation to conduct graduate study under the supervision of Drs. Marc Van Montagu (2013 World Food Prize Laureate) and the late Jeff Schell at Ghent University, Belgium, the first group that engineered Agrobacterium tumefaciens for plant genetic transformation. Her PhD study and postdoctoral research focused on the molecular mechanisms of the T-DNA transfer, establishing her as one of the pioneers in this corner stone area for the development of plant genetic transformation tools (Wang et al., Cell, 1984; Science 1987). Dr. Wang then served for 7 years as a project leader in genetic transformation of corn and soybean at ICI seeds (now Syngenta). In 1996, she accepted a position at Iowa State University and established the first public crop transformation facility, which provides genetic transformation services for corn, soybean, and rice.
Dr. Wang's research interests include novel plant genetic transformation and genome editing technologies, production systems for corn-based pharmaceutical and industrial products, and the function of Agrobacteriumnon-coding RNAs. Dr. Wang is currently a professor of Agronomy, Director of the Center for Plant Transformation and Co-PI of the Crop Bioengineering Consortium at Iowa State University.
Name: Mrs Luba Hickey